The Effect of Beetroot Juice on Vo₂Max in Futsal Athletes

Andi Nur Halisa; Ishak Bachtiar; Andi Ridwan

doi:10.26858/cpjok.v18i2.922

Authors

Andi Nur Halisa Universitas Negeri Makassar Author
Ishak Bachtiar Universitas Negeri Makassar Author
Andi Ridwan Universitas Negeri Makassar Author

DOI:

https://doi.org/10.26858/cpjok.v18i2.922

Keywords:

Beetroot Juice VO₂Max; Cardiorespiratory Endurance; Futsal Athletes; Sports Nutrition

Abstract

Cardiorespiratory endurance is a crucial component of performance in futsal because athletes are required to perform repeated high-intensity activities throughout a match. One nutritional strategy that has gained attention for improving aerobic capacity is beetroot juice (Beta vulgaris), which is rich in dietary nitrates and may enhance oxygen utilization during exercise. This study aimed to determine the effect of beetroot juice consumption on VO₂Max among futsal athletes of SMA Negeri 8 Makassar. This study employed a quantitative approach with a pre-experimental one-group pretest–posttest design. The sample consisted of 22 futsal athletes selected using a total sampling technique. Participants received 200 ml of beetroot juice for 16 intervention sessions over four weeks. VO₂Max was measured using the Multistage Fitness Test (Bleep Test) before and after the intervention. Data analysis included the Shapiro–Wilk normality test followed by the Paired Sample t-Test to compare pretest and posttest scores. The results showed a significant improvement in VO₂Max after beetroot juice supplementation. The mean VO₂Max increased from 44.50 ± 3.76 ml·kg⁻¹·min⁻¹ before the intervention to 54.61 ± 4.64 ml·kg⁻¹·min⁻¹ after the intervention, with an average increase of 10.11 ml·kg⁻¹·min⁻¹. Statistical analysis revealed a p-value of 0.000 (p < 0.05), indicating a significant effect of beetroot juice on VO₂Max. These findings suggest that the nitrate content in beetroot juice may enhance nitric oxide production, improve blood circulation, and increase oxygen utilization efficiency. Therefore, beetroot juice has potential as a natural ergogenic supplement to improve cardiorespiratory endurance in futsal athletes.

References

Aggil Ahmad Ramadhan, Raden Akhmad Aqtoo, Amrul Hayat, Qoyyum Saputra, Hikmal Soulisa, Tubagus Lucky Tri Iman Setiawan, Siti Nur Azizah, Nuri Maulida, Fauzan Amrullah Ramadhan, & Muhammad Faiza Akhira Ramadhan. (2025). Analysis of Endurance with Stamina on One of Tarkam Football Athlete. Multidisciplinary Scientific Journal , 2 (3), 71–77. Https://Doi.Org/10.69714/Bccdvn06

Aliman, R., Atiq, A., & Haetami, M. (2023). Physical fitness and performance characteristics in futsal athletes. Jurnal Pendidikan Jasmani Indonesia, 19(2), 115–124. https://journal.uny.ac.id/index.php/jpji

Anggraini, D., & Widodo, A. (2021). Analysis of VO₂Max as an indicator of cardiorespiratory endurance in athletes. Jurnal Keolahragaan, 9(1), 45–54. https://doi.org/10.21831/jk.v9i1.38567

Anggraini, FS, & Widodo, A. (2021). Analysis of Maximal Aerobic Capacity (Vo2max) in Unesa Football Athletes. Journal of Sports Health , 09 (04), 103–108.

Arimbi. (2020). Effect Of Beetroot Extract On Maximum Oxygen Volume Athletes .

Dieny, FF, Fitranti, DY, Panunggal, B., & Safitri, I. (2017). The Effect of Beetroot (Beta Vulgaris) Juice on Hemoglobin Levels and the Performance of Soccer Athletes. The Indonesian Journal of Nutrition , 5 (2), 119–126. https://Doi.Org/10.14710/Jgi.5.2.119-126

Bassett, D. R., & Howley, E. T. (2017). Limiting factors for maximum oxygen uptake and determinants of endurance performance. Medicine & Science in Sports & Exercise, 49(1), 70–84. https://doi.org/10.1249/MSS.0000000000001142

Buchheit, M., & Laursen, P. B. (2019). High-intensity interval training, solutions to the programming puzzle. Sports Medicine, 49(12), 1813–1830. https://doi.org/10.1007/s40279-019-01149-8

Creswell, J. W., & Creswell, J. D. (2018). Research design: Qualitative, quantitative, and mixed methods approaches (5th ed.). Sage Publications. https://us.sagepub.com

Dieny, F. F., Jauharany, F. F., Tsani, A. F. A., & Nissa, C. (2017). Dietary intake, nutritional status, and VO₂Max among adolescent athletes. Journal of Nutrition and Metabolism, 2017, 1–8. https://doi.org/10.1155/2017/1484652

Domínguez, R., Cuenca, E., Maté-Muñoz, J. L., García-Fernández, P., Serra-Paya, N., Estevan, M. C. L., Herreros, P. V., & Garnacho-Castaño, M. V. (2017). Effects of beetroot juice supplementation on cardiorespiratory endurance in athletes. Nutrients, 9(1), 43. https://doi.org/10.3390/nu9010043

Febriyanah, N., Dieny, FF, Nugraha, TW, & Afifah, DN (2023). The Effect of Pineapple Beet Juice (Beta Vulgaris L, Ananas Comosus) on Lactic Acid Levels in Soccer Athletes. Journal of Nutrition College, 12 (4), 327–335. https://Doi.Org/10.14710/Jnc.V12i4.41149

Field, A. (2018). Discovering statistics using IBM SPSS statistics (5th ed.). Sage Publications. https://uk.sagepub.com

Fraenkel, J. R., Wallen, N. E., & Hyun, H. H. (2019). How to design and evaluate research in education (10th ed.). McGraw-Hill Education. https://www.mheducation.com

Ghasemi, A., & Zahediasl, S. (2019). Normality tests for statistical analysis: A guide for non-statisticians. International Journal of Endocrinology and Metabolism, 17(2), e55432. https://doi.org/10.5812/ijem.55432

Hogwood, A. C., Anderson, K. C., Zevallos, J. O. De, Paterson, C., Weltman, A., & Allen, J. D. (2023). Limited Effects Of Inorganic Nitrate Supplementation On Exercise Training Responses : A Systematic Review And Meta ‑ Analysis. Sports Medicine - Open, 1–13. Https://Doi.Org/10.1186/S40798-023-00632-1

Jones, A. M., Thompson, C., Wylie, L. J., & Vanhatalo, A. (2018). Dietary nitrate and physical performance. Annual Review of Nutrition, 38, 303–328. https://doi.org/10.1146/annurev-nutr-082117-051622

Jones, A. M., Vanhatalo, A., Bailey, S. J., & Wylie, L. J. (2021). Dietary nitrate and exercise performance: Current evidence and future directions. Sports Medicine, 51(Suppl. 1), 1–15. https://doi.org/10.1007/s40279-021-01422-y

JULIA, E. (2021). Consumption Of Nitrate-Rich Beetroot Juice With Or Without Vitamin C Supplementation Increases The Excretion Of Urinary Nitrate, Nitrite, And N-Nitroso Compounds In Humans.

Kerksick, C. M., Wilborn, C. D., Roberts, M. D., Smith-Ryan, A., Kleiner, S. M., Jäger, R., Collins, R., Cooke, M., Davis, J. N., Galvan, E., Greenwood, M., Lowery, L. M., Wildman, R., Antonio, J., & Kreider, R. B. (2018). ISSN exercise and sports nutrition review update. Journal of the International Society of Sports Nutrition, 15(1), 38. https://doi.org/10.1186/s12970-018-0242-y

Mauludiyah, N., Hidayat, T., & Prasetyo, Y. (2023). Relationship between aerobic endurance and futsal performance among youth athletes. Jurnal SPORTIF, 9(2), 211–223. https://doi.org/10.29407/js_unpgri.v9i2.21459

Mauludiyah, N., Muhammad, HN, & Wibowo, S. (2023). Implementation of Station Rotation Learning in Physical Education Subjects on Activity Material for Fitness on Students' Agility Levels. Eduinovasi: Journal of Basic Educational Studies , 4 (1), 729–744. https://Doi.Org/10.47467/Edui.V4i1.6002

McMahon, N. F., Leveritt, M. D., & Pavey, T. G. (2017). The effect of dietary nitrate supplementation on endurance exercise performance in healthy adults: A systematic review and meta-analysis. Sports Medicine, 47(4), 735–756. https://doi.org/10.1007/s40279-016-0617-7

Mosher, S. L., Sparks, S. A., Williams, E. L., Bentley, D. J., & Mc Naughton, L. R. (2021). Ingestion of nitrate-rich beetroot juice improves repeated sprint performance. European Journal of Sport Science, 21(8), 1133–1141. https://doi.org/10.1080/17461391.2020.1820778

R. Aliman, A. Atiq, M. Haetami, W. Et Al. (2023). Journal of World Education. Journal of World Education , 3 (November), 67–78. Http://Jurnal.Stokbinaguna.Ac.Id/Index.Php/JURDIP/Article/View/2083

Raghuveer, G., Hartz, J., & Lubans, D. (2020). Cardiorespiratory fitness and sports performance in youth athletes. Progress in Cardiovascular Diseases, 63(4), 431–438. https://doi.org/10.1016/j.pcad.2020.05.004

Raghuveer, G., Hartz, J., Lubans, D. R., Takken, T., Wiltz, J. L., Mietus-Snyder, M., Perak, A. M., Baker-Smith, C., Pietris, N., & Edwards, N. M. (2020). Cardiorespiratory Fitness In Youth: An Important Marker Of Health: A Scientific Statement From The American Heart Association. Circulation, 142(7), E101–E118. Https://Doi.Org/10.1161/CIR.0000000000000866

Ramadhan, A. A., Putra, R., & Kurniawan, A. (2025). Physiological demands and performance indicators in youth futsal athletes. International Journal of Sports Science, 13(1), 55–64.

Ribeiro, N., Bef, A., Alves, D., Ferreira, K., Bf, C., Raimundo, J., Bef, F., Cristiane, A., Queiroz, C., Ignacio, D., Perez, V., Ariel, E., Munoz, A., Acef, B. M., Jose, C., & Acdef, B. (2021). Coaching & Exercise Physiology Beetroot Extract Improves Specific Performance And Oxygen Uptake In Taekwondo Athletes : A Double-Blind Crossover Study. 21(4), 12–19. Https://Doi.Org/10.14589/Ido.21.4.3

Sanrebayu, M. (2020). Potensi beetroot sebagai suplemen peningkat daya tahan atlet. Jurnal Gizi Olahraga Indonesia, 4(2), 89–98. https://ejournal.unesa.ac.id

Sanrebayu. (2020). The Effect of Beetroot Juice (Beta Vulgaris) on Hemoglobin Levels and VO2MAX Values of Badminton Athletes . Hasanuddin University.

Senefeld, J. W., Wiggins, C. C., Regimbal, R. J., Dominelli, P. B., Baker, S. E., Joyner, M. J., & Limberg, J. K. (2020). Ergogenic effect of nitrate supplementation on endurance performance. Medicine & Science in Sports & Exercise, 52(11), 2250–2261. https://doi.org/10.1249/MSS.0000000000002378

Thomas, D. T., Erdman, K. A., & Burke, L. M. (2016). Position of the Academy of Nutrition and Dietetics, Dietitians of Canada, and the American College of Sports Medicine: Nutrition and athletic performance. Journal of the Academy of Nutrition and Dietetics, 116(3), 501–528. https://doi.org/10.1016/j.jand.2015.12.006

Thomas, J. R., Nelson, J. K., & Silverman, S. J. (2022). Research methods in physical activity (8th ed.). Human Kinetics. https://us.humankinetics.com

Wylie, L. J., Kelly, J., Bailey, S. J., Blackwell, J. R., Vanhatalo, A., & Jones, A. M. (2019). Beetroot juice and intermittent exercise performance in trained athletes. International Journal of Sports Physiology and Performance, 14(4), 495–501. https://doi.org/10.1123/ijspp.2018-0310

Zahra, N., & Muhlisin, M. (2020). Kandungan antioksidan dan manfaat beetroot dalam olahraga. Jurnal Gizi Klinik Indonesia, 17(3), 156–164. https://doi.org/10.22146/ijcn.54758

Zahra, S., & Muhlisin, M.-. (2020). Nutrition for Adolescent Athletes. Journal of Applied Sports Science , 5 (1), 81–89. https://Doi.Org/10.17509/Jtikor.V5i1.25097